UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN KEMBANG TELANG (Clitoria ternatea L.) DENGAN METODE FRAP
Keywords:
Antioxidants, Telang Flower Leaf (Clitoria ternatea L.), FRAPs, Extract, SpectrophotometerAbstract
This research is about Antioxidant Activity Test of Kembang Telang (Clitoria ternatea L.) Leaf Ethanol Extract with Ferric Reducing Antioxidant Power (FRAP) Method. The purpose of this study was to determine the value of the antioxidant activity of the ethanol extract of butterfly pea leaves (Clitoria ternatea L.) using the FRAP method. The research method included extraction by maceration using 96% ethanol solvent, followed by antioxidant analysis using a Visible spectrophotometer at a wavelength of 689 nm. The results of the antioxidant activity test of the ethanol extract of butterfly pea leaves (Clitoria ternatea L.) were obtained with an IC50 value of 20.786 ± 0.014 ppm and ascorbic acid with an IC50 value of 8.131 ± 0.094 ppm indicating that the ethanol extract of butterfly pea leaves (Clitoria ternatea L.) has antioxidant activity in the category very strong.
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Amina, A. Muflihunna, Zainal A. 2016. Uji Aktivitas Antioksidan Fraksi Etil Asetat Daun Wungu (Graptophyllum pictum (Linn) Griff) Dengan Metode FRAP (Ferric Reducing Antioxidant Power). Jurnal ISSN 2085-4714. Vol. 8 No. 1, 39-44.
Apak R, Guclu K, Demirata B, Ozyurek M, Celik SE, Bektasuglu B, Berker KI, Ozyurt D. 2017. Comperative Evaluation Various Total Antioxidant Capacity Assays Applied Tophenoid CUPRAC assay. Molucules. Vol. 2 No. 17
Aprilia Kamaratih. 2019. Potensi Antioksidan Ekstrak Air Kembang Telang (Clitoria ternatea L.) Sebagai the Tradisional dalam Menghambat Peroksidasi Lipid. Karya Tulis Ilmiah. Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Pertanian Bogor. Bogor
Balai Teknologi Polimer. 2021. Pengujian Sampel Polimer dengan Alat UV-Vis. Diakses pada tanggal 28 September
Budiasih, K. S. 2017. Kajian Potensi Farmakologi Bunga Telang. Jurnal Pendidikan. Program Studi Kimia. Fakultas Matematika Dan Ilmu Pengetahuan Alam. Universitas Negeri Yogyakarta. Yogyakarta.
Cronquist, A. 1981. An Intregrated System of Classification of Flowering Plants, Columbia University Press. New York
Endang Hanani. 2015. Analisis Fitokimia. Penerbit Buku Kedokteran EGC. Jakarta.
Gea Krisnawati. 2017. Reaksi Radikal Bebas. On blogger Since. Diakses 28 september
Ginanjar Rifai, I Wayan Rai widarta, Komang Ayu Nociantri. 2018. Pengaruh Jenis Pelarut dan Rasio Bahan dengan Pelarut Terhadap Kandungan Senyawa Fenolik dan Aktivitas Antioksidan Ekstrak Biji Alpukat (Persea americana Mill.). Jurnal ITEPA, vol. 7 No.2.
Halvorsen, BL, Holte, Kari, Myhrstad, Mari CW, Barikmo, Hvattum Erlend, Remberg Siv Fagertun, Wold Anne-Birt, Haffner Karin, Baugerod Halvard, Andersen Lene Frost, Moskaug Jan, Jacobs David R, Blomhoff Rune. 2002. A Systematic Screening of Total Antioxodant in Dietary Plants. Journal of Nutrition.
Irmawati. 2015. Keajaiban Antioksidan. Penerbit Padi. Jakarta.
Iskandar Dodi. 2017. Perbandingan Metode Spektrofometri UV-Vis dan Iodimetri dalam Penentuan Asam Askorbat Sebagai Bahan Ajar Kimia Analitik. Mahasiswa jurusan teknologi pertanian berbasis Open-ended Experiment dan Problem Solving. Jurnal Teknologi Technoscientia. Universitas Negeri Yogyakarta ISSN: 1979-8415
Iqbal T. Muhamad. 2019. Aktivitas Antioksidan Ekstrak Etanol dan Profil Metabolit Sekunder Tanaman Kembang Telang (Clitoria ternate L.). Karya Tulis Ilmiah. Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Pertanian Bogor. Bogor
Kattappagari, KK., CS, Teja., RK, Kommalapati., C, Poosaria., SR, Gontu., BVR, Reddy. 2015. Role of Antioxidant in Facilitating the Body Functions. Journal of Orofacial Sciences. Vol. 7 No. 2, 71-75
Kim, O. S. 2005. Radical Scaenging Capacity and Antioxidant Activity of The Vitamin Fraction in rice bran. J Food Sci. ol 3 No 208-213
Kosai P., Kanjana S., Kanita J & Wannee J. 2015. Review On Ethnomedicinal Uses of Memory Boosting Herb, Butterfly Pea, Clitoria ternatea. Journal of Natural Remedies. Vol. 15 No. 2
Lembaga Ilmu Pengetahuan Indonesia. 2021. Analisa Absorbansi Larutan dengan Spektrofotometer UV-Vis. Diakses 28 september.
Magfira. 2018. Analisis Penghambatan Ekstrak Etanol Batang Kembang Bulan (Tithonia diversifolia) Terhadap Reaksi Oksidasi Dari Radikal Bebas Dengan Metode DPPH ABTS dan FRAP. Skripsi. Program Studi Farmasi. Fakultas Farmasi Universitas Hasanuddin Makassar. Makassar
Muslikha Nisa. 2020. Uji Aktivitas Antioksidan Menggunakan Metode FRAP Pada Fraksi Etil Asetat Ekstrak Etanol Daun Jambu Mete (Anacardium ocidentale L.). Karya Tulis Ilmiah. Sekolah Tinggi Ilmu Kesehatan Nasional Surakarta. Surakarta
Marjoni, Mhd Riza. 2016. Dasar-dasar Fitokimia untuk Diploma III Farmasi. Trans Info Media Press. Jakarta
Maryam St., Baits Muzakir., Nadia Ainun. 2015. Pengukuran Aktivitas Antioksidan Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) Menggunakan Metode FRAP (Ferric Reducing Antioxidant Power). Jurnal Fitofarmaka Indonesia. Vol. 2 No. 2
Najib Ahmad. 2018. Ekstraksi Senyawa Bahan Alam. Ed.1, Cet. 1.Yogyakarta
Nurhayati, T.D., Aryanti dan Nurjannah. 2009. Kaiian Awal Potensi Ekstrak Spons sebagai Antioksidan. Jurnal Kelautan Nasional. Vol 2. No. 2.
Putri, Dyan M.S. 2019. Konservasi Tumbuhan Obaat di Kebun Raya Bali. Bulletin Udayana Mengabdi. Vol.18 No. 3, 139-146
Rais, Icwan Ridwan. 2015. Isolasi dan Penentuan Kadar Flavonoid Ekstrak Etanolik Herba Sambiloto (Andrographis Paniculata (burm. F.) Ness). Jurnal Fakultas Farmasi Universitas Ahmad Dahlan. Vol. 5 No. 1
Santoso Umar. 2016. Antioksidan Pangan. Gadjah Mada University Press. Yogyakarta. Yogyakarta
Sayuti Kesuma., Rina Yenrina. 2015. Antioksidan Alami dan Sintetik, Andalas Universitas Press. Padang. Padang
Setiawan, F., Oeke, Y., Ade Kurniawan. 2018. Uji Aktivitas Antioksidan Ekstrak Etanol Kayu Secang (Caesalpinia sappan) Menggunakan Metode DPPH, ABTS, dan FRAP. Jurnal Penelitian. Fakultas Farmasi. Universitas Surabaya. Surabaya
Shen, Y et al. 2016. Butterfly Pea (Clitoria ternatea) Seed and Petal Extracts Decreased HEp-2 Carcinoma Cell Viability. International Journal of Food Science and Techonology, 51, pp. 1860-1868
Triyanto. 2016. Manfaat dan Khasiat Bunga Telang untuk Kesehatan Mata. Jurnal EduMatSains. Vol. 4 No. 2
Utami, Nofi Fajar. 2020. Buku Reverensi Hasil Riset Potensi Antioksidan dari Kopi Robusta dari 9 Daerah di Pulau Jawa. Lembaga Penelitian dan Pengabdian pada Masyarakat. Universitas Pakuan Bogor. Bogor
Wahyuni, Dian Ari. 2018. The Unity Color Of Kembang Telang. KaryaTulis Ilmiah. Institut Seni Indonesia Denpasar. Denpasar
Wahyuni, Indri Reski. 2015. Validasi Metode Analisis Uji Aktivitas Antioksidan Ekstrak N-Heksan, Etil Asetat, Etanol 70% Umbi Talas Ungu (Clitoria esculenta L. Schoot) Dengan Metode DPPH, CUPRAC dan FRAP Secara Spektrofotometri UV-Vis. Skripsi. Fakultas Kedokteran dan Ilmu Kesehatan. Universitas Islam Negeri Alauddin Makassar. Makassar
Winarti N., Rissa L, V., Jatmiko, S. 2020. Uji Aktivitas Antioksidan Ekstrak Bunga Telang (Clitoria ternatea L.) dengan Pelarut etanol dan Etil Asetat menggunakan Metode FRAP (Frric Reducing Antioxidant Power) Karya Tulis Ilmiah. Program Studi Farmasi. Sekolah Tinggi Ilmu Kesehatan Nasional Surakarta. Surakarta
Wulandari, Debora Anggi. 2017. Pengaruh Jenis Gula Pada Aktivitas Antioksidan Dan Potensi Probiotik Kombucha Teh Putih Dengan Penambahan Lactobacillus pentosus A22. Other Thesis, Universitas Katolik Soegijapranata Semarang. Semarang.
Yanlinastuti dan Syamsul Fatimah. 2016. Pengaruh Konsentrasi Pelarut Untuk Menentukan Kadar Zirikonium dalam Paduan U-Zr dengan menggunakan Metode Spektrofotometer UV-Vis. Pin Pengolahan Instalasi Nuklir, ISSN 1979-2409 hal 23.
Yuslianti, Euis Reni. 2018. Pengantar Radikal Bebas dan Antioksidan. Edisi 1. Cetakan 1. Yogyakarta
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