Respon Anatomi Tanaman Bunga Matahari (Helianthus annuus L.) Terhadap Tembaga
Keywords:
Anatomi, Helianthus annuus L., tembagaAbstract
Penelitian ini bertujuan untuk mengetahui respon anatomis tanaman bunga matahari (H. annuus L.) terhadap tembaga dan dilakukan dengan menggunakan Rancangan Acak Lengkap yang terdiri dari 4 konsentrasi perlakuan logam Cu yaitu P0: 0 mg/L, P1: 100 mg/L, P2: 200 mg/L dan P3: 500 mg/L, dengan 5 ulangan untuk setiap perlakuan. Penelitian ini dilakukan dengan merendam biji bunga matahari kultivar dalam wadah berisi air hangat selama semalam kemudian ditanam pada polybag. Tanaman dipelihara secara intensif hingga berumur 2 minggu setelah tanam dan dilakukan aplikasi Cu. Logam Cu yang digunakan berupa garam CuSO4.5H2O. Setelah aplikasi Cu, dilakukan pengukuran pertumbuhan tanaman, pembuatan dan pengamatan preparat anatomi akar dan daun menggunakan optilab. Parameter penelitian meliputi parameter anatomis (akar terdiri dari tebal sel epidermis, tebal jaringan korteks, dan tebal berkas pengangkut; daun meliputi tebal sel epidermis atas dan bawah, tebal jaringan palisade, tebal jaringan spons, tebal berkas pengangkut, dan indeks stomata). Data hasil pengamatan disajikan dalam bentuk gambar dan pengukuran akan dianalisis menggunakan analisis sidik ragam dan diuji lanjut dengan Duncan Multiple Range Test dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian Cu berpengaruh terhadap anatomi tanaman bunga matahari dengan adanya kerusakan sel-sel epidermis dan korteks akar, penebalan berkas pengangkut akar, sel epidermis atas dan bawah daun, tebal jaringan palisade, jaringan spons, dan penebalan berkas pengangkut pada daun dan stomata daun bertipe fanerofor.
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