ANALISIS KANDUNGAN SENYAWA KIMIA DARI EKSTRAK KLOROFORM DAUN AKAR BULU (merremia vitifolia) MENGGUNAKAN GC-MS
Keywords:
Akar bulu, ekstrak kloroform, GCMS.Abstract
Tujuan dari penelitian ini adalah untuk mengetahui kandungan senyawa kimia dari ekstrak kloroform daun akar bulu (Merremia Vitifolia) menggunakan GCMS. Metode yang digunakan pada penelitian ini adalah preparasi sampel, maserasi sampel, dimana maserasi menggunakan dua metode yaitu metode secara langsung yang menggunakan pelarut kloroform dalam proses maserasi dan metode secara tidak langsung menggunakan dua pelarut yaitu pada maserasi menggunakan pelarut etanol 96% dan ekstraksi cair-cair menggunakan pelarut kloroform. Proses pengentalan dilakukan menggunakan alat destilasi, kemudian dianalisis kandungan senyawa kimia menggunakan GCMS. Hasil penelitian yang diperoleh dengan indeks kemiripan diatas 90% didapatkan 14 senyawa dan diatas 80-89% indeks kemiripan didapatkan 8 senyawa serta senyawa dengan indeks kemiripan 70-79 % didapatkan 5 senyawa. Senyawa yang dihasilkan dengan indeks kemiripan paling tinggi yaitu senyawa Hexanedioic acid, bis(2-ethylhexyl) ester (97%) dan senyawa yang paling dominan adalah senyawa2-hexadecen-1-ol, 3,7,11,15-tetramethyl-, [r-[r*,r*-(e)]]- dengan persen areanya sebesar 32.92.
Kata kunci: Akar bulu, ekstrak kloroform, GCMS.
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References
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[4] Sukarti, S. (2016). Screening Fitokimia Ekstrak Polar Daun Tumbuhan Tali Gurita (Family Cucurbitaceae) Yang Berpotensi Sebagai Antidiabetes. Journal of Mathematics and Natural Sciences, 7(2), 9-15.Sumanta, A., Gourange Das, Sanjoy Kumar D. 2011. Roles Of Flavonoid In Plants. Int J Pharma Sci Tech. Vol-6, Issue-1.
[5] Jaafar, F.M., Osman, C. P., Ismail, N. H. Dan Awang, K. (2007). Analysis Of Essential Oils Of Leaves, Stems, Flowers And Rhizomes Of Etlingera Elatior (Jack) R. M. S. Smith. The Malaysian Jurnal Of Analytical Sciences, 11 (1), 269-273.
[6] Harborne, J. B., 1987. Metode Fitokimia. Penuntun Cara Modern Menganalisis Tumbuhan. Terjemahan K. Padmawinata & I. Soediro, Penerbit ITB, Bandung.
[7] Putu, N., Kusuma, R., & Muderawan, I. W. (2016). Analisis Kandungan Kimia Ekstrak Daun Sirih Hijau ( Piper Betle ) dengan Gc-MS.
[8] Yulianti, K. (2020). Analisis Kadar Dan Identifikasi Gugus Fungsi Senyawa Flavonoid dari Ekstrak Kloroform Daun Akar Bulu (Merremia vitifolia). Skripsi. Fakultas Sains. Universitas Cokroaminoto Palopo.
[9] Marjoni, R. (2016). Dasar-Dasar Fitokimia Untuk Diploma I I. Jakarta: Cv. Trans Info Media.
[10] Ningsih, AW., Hanifa, I, Dan Hisbiyah, A. (2020). Pengaruh Perbedaan Metode Ekstraksi Rimpang Kunyit (Curcuma Domestica) Terhadap Rendemen Dan Skrining Fitokimia. Journal Of Pharmaceutical Care Anwar Medika. Vol 2(2):49- 57.
[11] Aisyah, T. S., & Asnani, A. (2012). Kajian Sifat Fisikokimia Ekstrak Rumput Laut Coklat (Sagarsum duplicatum) Menggunakan Berbagai Pelarut dan Metode Ekstraksi. Kajian Sifat Fisikokimia Ekstrak Rumput Laut, 6(1), 22.
[12] Sa'adah, H., & Nurhasnawati, H. (2017). Perbandingan Pelarut Etanol Dan Air Pada Pembuatan Ekstrak Umbi Bawang Tiwai (Eleutherine Americana Merr) Menggunakan Metode Maserasi. Jurnal ilmiah manuntung, 1(2), 149-153.
[13] Rahmiyani, I. (2020). Isolasi dan Identifikasi Senyawa Minyak Atsiri Daun Gamal (Gliricidia sepium [Jacq] Walp). Jurnal Farmasi Udayana, 134-143.
[14 Agoramoorthy M., Chandrasekaran V., Venkatesalu M.J.H. (2007). Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Braz J Microbiol; 38: 739– 742.
[15] Zayed MZ. (2016). Benedict Samling, Phytochemical Constituents of the Leaves of Leucaena Leucoephala From Malaysia. Int J Pharm Pharm Sci. 8(12):174-179.
[16]Euphorbiaceae, W., Sudha, T., Chidambarampillai, S., & Mohan, V. R. (2013). GC-MS Analysis of Bioactive Components of Aerial parts of Fluggea. 3(05), 126–130.
[17] da Costa, G. A. F.; Morais, M. G.; Saldanha, A. A.; Assis Silva, I. C.; Aleixo, Á. A.; Ferreira, J. M. S.; Soares, A. C.; Duarte-Almeida, J. M.; Lima, L. A. R. dos S. (2015). Evidence-Based Complement. Altern. Med. 2015, 1–8.
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[22] Sianipar, N.F., Purnamaningsih, R., Darwati, L., & Laurent, D. (2016). Gas Chromatography-Mass Spectrometry (GC-MC) Analisys of Phytochemicals of First Generation Gamma-Irradiated Typhonium Flagelliforme Lodd. Mutants. Jutnal Teknologi, 78(10-4). 1-7.
[23] Julianus, J., & Luckyvano, E. (2014). Sintesis asam sinamat dari benzaldehida dan asam malonat dengan katalis dietilamina. Angewandte Chemie International Edition, 6(11), 951–952., 11(1), 1–6.
[24] National Center for Biotechnology Information (2022). PubChem Compound Summary for CID 240, Benzaldehyde. Retrieved July 01, 2022 from https://pubchem.ncbi.nlm.nih.gov/compound/Benzaldehyde.
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Published
2023-07-05
How to Cite
[1]
sukarti sukarti, “ANALISIS KANDUNGAN SENYAWA KIMIA DARI EKSTRAK KLOROFORM DAUN AKAR BULU (merremia vitifolia) MENGGUNAKAN GC-MS”, CJCS, vol. 5, no. 2, pp. 30–38, Jul. 2023.
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